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Highlighted Features

• Multi-State Experiments: Can analyze solids, liquids, and semi-solids. 
• High-Field Magnet: Equipped with a 14.1 Tesla magnet (600 MHz proton frequency) for high sensitivity and resolution.
• Solid-State: Has fast-spinning solid-state NMR probes (up to 40 kHz MAS) to resolve fine details in solids from proteins to polymers.
• Diffusion: Has a broad-band diffusion NMR probe capable of tracking molecular motions and quantifying diffusion coefficients in liquids and polymers, ideal for studying battery materials and pharmaceuticals.
• Liquid-state: The broad-band diffusion NMR probe also functions as a state-of-the-art liquid-state NMR probe, enabling molecular-level studies of liquids and small molecules of various compositions. 
• Flexible Temperature Range: Probes can operate from –100 °C to 180 °C for dynamic studies under varied conditions. 
• Diverse Applications: Supports research in diverse areas including structural biology (e.g., proteins), materials science (e.g., battery materials), and polymer chemistry.

Explore Detailed Specifications

Location and Contact Information

• Location: CCNY, CDI G-436
• Facility Manager: Dr. Jianqin Zhuang

Hours

• Open 24/7 to all qualified users.

User Fees

Effective as of 09/01/2025

Images

Instrument specifications

Description

The Bruker Avance Neo 600 MHz NMR spectrometer is a mixed-use instrument that is capable of carrying out NMR experiments in solution, semi-solid, as well as solid-state. The main focus, however, is in solid state research. The instrument operates with an actively shielded 14.7 T superconducting magnet (600 MHz for ¹H) and a compact 5-Gauss stray-field radius of 6 ft. The system includes three RF channels that support advanced multi-nuclear, multi-dimensional experiments in structural biology, polymer science, and materials research. Most solid-state experiments are performed under magic-angle spinning (MAS) at 54.7°, which averages out dipolar interactions and sharpens spectral features. Our fastest MAS probe spins up to 40 kHz. All solid-state probes are HXY-type MAS probes with broadband X channels (³¹P to ¹⁵N). Current research includes isotopically labeled proteins, plant and fungal materials, lipid and phosphorylation studies, and energy-storage materials involving ⁷Li and ¹¹B. In addition, the system is equipped with a Bruker 5 mm Diffusion Broadband (DiffBB) probe for solution state and DOSY experiments. The DiffBB probe functions as a state-of-the-art broadband liquid-state NMR probe, as the X-broadband channel enables observation of nuclei ranging in frequency from ³¹P down to ¹⁵N. It is optimized for pulsed-field-gradient (PFG) NMR, enabling precise measurement of molecular motion across a wide range of viscosities and molecular sizes. It provides two RF channels (H1/F19 and X), a water-cooled single-axis gradient coil delivering up to 1700 G/cm, and a broad –100 °C to 180 °C temperature range. This setup is well suited for studying molecular diffusion, interactions, and dynamics in complex systems. Current research focuses on measuring ion transport properties in electrolytes and distinguishing dissolved species based on their diffusion coefficients, enabling detailed insight into molecular mobility and interaction dynamics.

Services

Multi-dimensional, multi-nuclear solid state NMR research on protein

Solid state NMR research on materials, such as natural or synthetic polymers, and energy storage material

Study of Semi-solid materials such as biological tissues or swollen polymers

Probes

1.6 mm FastMAS Probe: A 3 channel (HXY) MAS probe that spins up to 40 kHz.  The probe could offer higher resolution with reduced dipolar coupling because of the high spinning speed.  Despite the small size of sample rotor (1.6 mm), the probe has excellent sensitivity.

3.2 mm T3HXY probe: A 3 channel (HXY) MAS probe that spins up to 25 kHz.  It has a traditional solenoid coil with excellent sensitivity.

Broadband Diffusion Probe (DiffBB): A 2 channel (H1/F19, X) 5-mm probe with a strong z-axis gradient up to 1700 G/cm, enabling PFG diffusion measurements down to 1 × 10⁻¹⁴ m²/s. It alsosupports multi-dimensional solution NMR experiments and operates across a –100 °C to 180 °C temperature range, suitable for research in polymers, pharmaceuticals, and battery materials.

Recent Publication

Michael J. Keating, Elijah Bernard, Martina Hove, Ho Martin Yuen, Mehreen Mughal, Surabh S. KT, James F. Wishart, Sharon Lall-Ramnarine, Robert J. Messinger, and Elizabeth J. Biddinger, Influence of Ether-Functionalized Pyrrolidinium Ionic Liquids on Properties and Li+ Cation Solvation in Solvate Ionic Liquids, The Journal of Physical Chemistry C 2025 129 (24), 10802-10814 DOI: 10.1021/acs.jpcc.5c01403

E. Camacho, Y. Dong, C. Chrissian, R. J. B. Cordero, R. Saravia, Y. Anglero-Rodriguez, D. F. Q. Smith, E. Jacobs, I. Hartshorn, J. A. Patiño-Medina, M. DePasquale, A. Dziedzic, A. Jedlicka, B. Smith, G. Mlambo, A. Tripathi, N. A. Broderick, R. E. Stark, G. Dimopoulos, A. Casadevall, “Dietary L-3,4-dihydroxyphenylalanine (L-DOPA) augments cuticular melanization in Anopheles mosquitoes while reducing their lifespan and malaria parasite burden,” Nature Commun. 16, 8011 (2025).

A. Ankur, J. R. Yarava, I. Gautam, F. J. Scott, F. Mentink-Vigier, C. Chrissian, L. Xie, D. Roy, R. E. Stark, T. L. Doering, P. Wang, T. Wang, “Polymorphic α-Glucans as Structural Scaffolds in Cryptococcus Cell Walls for Chitin, Capsule, and Melanin: Insights from 13C and 1H Solid-State NMR,” Angew. Chem,, 2025, e202510409. 

G. C. Arya, E. Wassel, E. Manasherova, R. E. Stark, and H. Cohen, “Exocarp-specific expression of a fungal cutinase in tomato fruits alters cuticle ultrastructure, chemistry and nanomechanics,” Plant Physiol., 199: kiaf376 (2025). 

K. Dastmalchi, V.C. Phan, S. Chatterjee, B. Yu, M. Figueras, O. Serra, and R. E. Stark, “A Comprehensive Approach to Phytochemical Analysis of Macromolecular Composites that Protect Tubers: case studies in suberized potato periderm tissues,” Phytochemistry Rev. 24, 909-925 (2024). 

E. Jacobs, C. Chrissian, S. Rankin-Turner, M. Wear, E. Camacho, N. Broderick, C. McMeniman, R. E. Stark, A. Casadevall, “Cuticular profiling of insecticide resistant Aedes aegypti,” Sci. Reports, 13, 10154 (2023).

Jian Zhang, Jiayan Shi, Leo W. Gordon, Nastaran Shojarazavi, Xiaoyu Wen, Yifan Zhao, Jianjun Chen, Chi-Cheung Su, Robert J. Messinger, and Juchen Guo, "Performance Leap of Lithium Metal Batteries in LiPF6 Carbonate Electrolyte by a Phosphorus Pentoxide Acid Scavenger", ACS Applied Materials & Interfaces 2022 14 (32), 36679-36687, DOI: 10.1021/acsami.2c09267

R. P. Baker, C. Chrissian, R. E. Stark and A. Casadevall, “Cryptococcus neoformans melanization incorporates multiple catecholamines to produce polytypic melanin,” J. Biol. Chem., Online 12/20/21, 298(1), 101519 (2021).

C. Chrissian, C. Lin, E. Camacho, A. Casadevall, A. Neiman, and R. E. Stark, “Unconventional constituents in the fungal cell walls of Saccharomyces cerevisiae and Cryptococcus neoformans,” J. of Fungi, 6, 329-345 (2020).

C. Chrissian, E. Camacho, J. E. Kelly, H. Wang, A. Casadevall, and R. E. Stark, “Solid-state NMR spectroscopy identifies three classes of lipids in C. neoformans melanized cell walls and whole fungal cells,” J. Biol. Chem., 295, 15083-15096 (2020).

C. Chrissian, E. Camacho, M.S. Fu, R. Prados-Rosales, S. Chatterjee, R. J. B. Cordero, J. K. Lodge, A. Casadevall, and R. E. Stark, “Melanin deposition in two Cryptococcus species depends on cell-wall composition and flexibility,” J. Biol. Chem., 295, 1815-1828 (2020). 

S. Chatterjee, R. Prados-Rosales, S. Tan, V. C. Phan, C. Chrissian, B. Itin, H. Wang, A. Khajo, R. S. Magliozzo, A. Casadevall, and R. E. Stark, “The melanization road more traveled by: precursor substrate effects on melanin synthesis in cell-free and fungal cell systems,” J. Biol. Chem., 293, 20157-20168 (2018).